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muc 2 protein targeting rabbit antibodies  (Servicebio Inc)

 
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    Structured Review

    Servicebio Inc muc 2 protein targeting rabbit antibodies
    Oral administration of A. actinomycetemcomitans was associated with aggravated intestinal barrier impairment and elevated levels of inflammatory mediators in mice with colitis. (A) Relative mRNA expression levels of pro-inflammatory cytokines in colon tissue were normalized to Gapdh (2 -ΔΔCt method). (B) Serum concentrations of IL-6, IL-1β, and TNF- α measured by ELISA. (C) Representative immunofluorescence images of colon sections stained for the tight junction protein ZO-1 (green) and the <t>mucin</t> <t>MUC-2</t> (red). Nuclei were counterstained with DAPI (blue). Scale bars: 200 μm. Quantification of ZO-1 and MUC-2 signal intensity was performed on at least 9 non-overlapping fields per mouse using ImageJ software. Data are representative of two independent experiments. Data are shown as mean ± SEM. Statistical analysis was performed using two-sided one-way ANOVA followed by the least significant difference (LSD) post–hoc test or two-sided unpaired Student’s t-test and Dunnett T3 (uneven variance). * p < 0.05, ** p < 0.01, *** p < 0.001.
    Muc 2 Protein Targeting Rabbit Antibodies, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/muc 2 protein targeting rabbit antibodies/product/Servicebio Inc
    Average 86 stars, based on 1 article reviews
    muc 2 protein targeting rabbit antibodies - by Bioz Stars, 2026-06
    86/100 stars

    Images

    1) Product Images from "Aggregatibacter actinomycetemcomitans exacerbates colitis and perturbs the gut microbiota in a murine model​"

    Article Title: Aggregatibacter actinomycetemcomitans exacerbates colitis and perturbs the gut microbiota in a murine model​

    Journal: Journal of Oral Microbiology

    doi: 10.1080/20002297.2026.2613536

    Oral administration of A. actinomycetemcomitans was associated with aggravated intestinal barrier impairment and elevated levels of inflammatory mediators in mice with colitis. (A) Relative mRNA expression levels of pro-inflammatory cytokines in colon tissue were normalized to Gapdh (2 -ΔΔCt method). (B) Serum concentrations of IL-6, IL-1β, and TNF- α measured by ELISA. (C) Representative immunofluorescence images of colon sections stained for the tight junction protein ZO-1 (green) and the mucin MUC-2 (red). Nuclei were counterstained with DAPI (blue). Scale bars: 200 μm. Quantification of ZO-1 and MUC-2 signal intensity was performed on at least 9 non-overlapping fields per mouse using ImageJ software. Data are representative of two independent experiments. Data are shown as mean ± SEM. Statistical analysis was performed using two-sided one-way ANOVA followed by the least significant difference (LSD) post–hoc test or two-sided unpaired Student’s t-test and Dunnett T3 (uneven variance). * p < 0.05, ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: Oral administration of A. actinomycetemcomitans was associated with aggravated intestinal barrier impairment and elevated levels of inflammatory mediators in mice with colitis. (A) Relative mRNA expression levels of pro-inflammatory cytokines in colon tissue were normalized to Gapdh (2 -ΔΔCt method). (B) Serum concentrations of IL-6, IL-1β, and TNF- α measured by ELISA. (C) Representative immunofluorescence images of colon sections stained for the tight junction protein ZO-1 (green) and the mucin MUC-2 (red). Nuclei were counterstained with DAPI (blue). Scale bars: 200 μm. Quantification of ZO-1 and MUC-2 signal intensity was performed on at least 9 non-overlapping fields per mouse using ImageJ software. Data are representative of two independent experiments. Data are shown as mean ± SEM. Statistical analysis was performed using two-sided one-way ANOVA followed by the least significant difference (LSD) post–hoc test or two-sided unpaired Student’s t-test and Dunnett T3 (uneven variance). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Software



    Similar Products

    muc 2 protein targeting rabbit antibodies  (Servicebio Inc)
    86
    Servicebio Inc muc 2 protein targeting rabbit antibodies
    Oral administration of A. actinomycetemcomitans was associated with aggravated intestinal barrier impairment and elevated levels of inflammatory mediators in mice with colitis. (A) Relative mRNA expression levels of pro-inflammatory cytokines in colon tissue were normalized to Gapdh (2 -ΔΔCt method). (B) Serum concentrations of IL-6, IL-1β, and TNF- α measured by ELISA. (C) Representative immunofluorescence images of colon sections stained for the tight junction protein ZO-1 (green) and the <t>mucin</t> <t>MUC-2</t> (red). Nuclei were counterstained with DAPI (blue). Scale bars: 200 μm. Quantification of ZO-1 and MUC-2 signal intensity was performed on at least 9 non-overlapping fields per mouse using ImageJ software. Data are representative of two independent experiments. Data are shown as mean ± SEM. Statistical analysis was performed using two-sided one-way ANOVA followed by the least significant difference (LSD) post–hoc test or two-sided unpaired Student’s t-test and Dunnett T3 (uneven variance). * p < 0.05, ** p < 0.01, *** p < 0.001.
    Muc 2 Protein Targeting Rabbit Antibodies, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/muc 2 protein targeting rabbit antibodies/product/Servicebio Inc
    Average 86 stars, based on 1 article reviews
    muc 2 protein targeting rabbit antibodies - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Oral administration of A. actinomycetemcomitans was associated with aggravated intestinal barrier impairment and elevated levels of inflammatory mediators in mice with colitis. (A) Relative mRNA expression levels of pro-inflammatory cytokines in colon tissue were normalized to Gapdh (2 -ΔΔCt method). (B) Serum concentrations of IL-6, IL-1β, and TNF- α measured by ELISA. (C) Representative immunofluorescence images of colon sections stained for the tight junction protein ZO-1 (green) and the mucin MUC-2 (red). Nuclei were counterstained with DAPI (blue). Scale bars: 200 μm. Quantification of ZO-1 and MUC-2 signal intensity was performed on at least 9 non-overlapping fields per mouse using ImageJ software. Data are representative of two independent experiments. Data are shown as mean ± SEM. Statistical analysis was performed using two-sided one-way ANOVA followed by the least significant difference (LSD) post–hoc test or two-sided unpaired Student’s t-test and Dunnett T3 (uneven variance). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Journal of Oral Microbiology

    Article Title: Aggregatibacter actinomycetemcomitans exacerbates colitis and perturbs the gut microbiota in a murine model​

    doi: 10.1080/20002297.2026.2613536

    Figure Lengend Snippet: Oral administration of A. actinomycetemcomitans was associated with aggravated intestinal barrier impairment and elevated levels of inflammatory mediators in mice with colitis. (A) Relative mRNA expression levels of pro-inflammatory cytokines in colon tissue were normalized to Gapdh (2 -ΔΔCt method). (B) Serum concentrations of IL-6, IL-1β, and TNF- α measured by ELISA. (C) Representative immunofluorescence images of colon sections stained for the tight junction protein ZO-1 (green) and the mucin MUC-2 (red). Nuclei were counterstained with DAPI (blue). Scale bars: 200 μm. Quantification of ZO-1 and MUC-2 signal intensity was performed on at least 9 non-overlapping fields per mouse using ImageJ software. Data are representative of two independent experiments. Data are shown as mean ± SEM. Statistical analysis was performed using two-sided one-way ANOVA followed by the least significant difference (LSD) post–hoc test or two-sided unpaired Student’s t-test and Dunnett T3 (uneven variance). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: The ZO-1 and MUC-2 protein-targeting rabbit antibodies (Servicebio, China) were applied to the 4 μm-thick tissue sections, which were subsequently incubated overnight at 4 °C, washed with phosphate-buffered saline, and then treated with anti-rabbit fluorescent secondary antibodies (Servicebio, China) at 37 °C for 1 h. Following washing, the tissue nuclei were re-stained with 4-methyl-6-methyl-2-phenylindole (DAPI).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Software